International Journal of Laser Dentistry

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2015 | August | Volume 5 | Issue 2

ORIGINAL ARTICLE

Natalino Lourenço Neto, Ana P Fernandes, Vivien T Sakai, Bella LC Ishikiriama, Fernanda V Oliveira, Carlos F Santos, Maria AAM Machado

Low-level Laser Therapy Effects on Pulp Cells from Human Primary Teeth

[Year:2015] [Month:] [Volume:5] [Number:2] [Pages:5] [Pages No:33 - 37]

PDF  |  DOI: 10.5005/jp-journals-10022-1070  |  Open Access |  How to cite  | 

Abstract

The aim of this study was to verify the influence of different energy densities of low-level laser therapy (LLLT) on the viability and proliferation of pulp fibroblasts of human primary teeth (HPF). Cultures of fibroblast were irradiated with LLLT according to experimental groups: I (1.2 J/cm2), II (2.5 J/cm2), III (3.7 J/cm2), IV (5.0 J/cm2), V (6.2 J/cm2), and VI (not irradiated). Cell viability was determined 6 and 24 hours after of irradiation using 3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2H- tetrazolium bromide (MTT) assay. Cell proliferation was assessed through sulforhodamine B (SRB) assay after 24, 48, and 72 hours of irradiation. Data were submitted to two-way analysis of variance (ANOVA) and Tukey test (p < 0.05). After 6 hours of irradiation, group III exhibited the greatest cell viability compared with other groups. After 24 hours, group I exhibited the smallest absorbance value compared with group VI. To cell proliferation, 24 and 48 hours after irradiation, all groups showed similar results to those of group VI. After 72 hours, group I showed lower cell proliferation than the others. Group V exhibited results very similar to those of group VI. This study suggests that different energy densities of LLLT influenced the viability and proliferation of HPF.

ORIGINAL ARTICLE

Abhishek Gautam

Semiconductor Diode Laser provides Desirable Smile: A Clinical and Histological Study with 1-year Follow-up

[Year:2015] [Month:] [Volume:5] [Number:2] [Pages:5] [Pages No:38 - 42]

PDF  |  DOI: 10.5005/jp-journals-10022-1071  |  Open Access |  How to cite  | 

Abstract

Aim: The semiconductor diode (SCD) laser is a compact, costeffective, and versatile tool for performing soft tissues applications. The advantage of laser includes a relatively bloodless operating field, sterilization of the wound site, minimal swelling and scaring, reduction of surgical time, and less postoperative pain to the patients in dentistry. This technique is successfully used to evaluate the effects for removal of gingival melanin hyperpigmentation and to assess the color of gingiva pain and wound healing, along with the appearance of gingival repigmentation by clinical and histological examination, in the present study. Materials and methods: For this purpose, five patients were studied which includes four females and one male, aged between 19 and 40, where four patients were nonsmokers and one was a smoker and found periodontally healthy and had no systemic diseases. Results: The SCD laser found effective in removing gingival melanin pigmentation, and no bleeding with any significant pain was reported by patients. However, repigmentations were observed with Fontana–Masson staining. Conclusion: These result pointed out that SCD laser is good and safe for removal of pigmented gingiva without local anesthesia. The postoperative period is comfortable for the patient, and healing is fast and good. Clinical significance: Semiconductor diode laser is an easy and effective tool for removal of gingiva hyperpigmentation. Ablation of gingival hyperpigmented areas was accomplished without any bleeding complication or slight pain, and no pain was observed which provide clean field during time of procedures, and there was uneventful healing without any complication. No recurrence or slight recurrence of pigmentation had been found in 12-month follow-up.

RESEARCH ARTICLE

Mohamad T Kiani, Bahman Derakhshan, Mina Abasi, Shahrooz Shafahifard, Mohammad J Kharazifard

Effect of Low-level Laser Therapy on Neurosensory Disorders Recovery following the Bilateral Sagittal Split Ramus Osteotomy

[Year:2015] [Month:] [Volume:5] [Number:2] [Pages:7] [Pages No:43 - 49]

PDF  |  DOI: 10.5005/jp-journals-10022-1072  |  Open Access |  How to cite  | 

Abstract

Introduction: Bilateral sagittal split osteotomy (BSSO) is a technique commonly used to correct mandibular disproportions, while neurosensory disorders are common following this technique. Low-level laser irradiation has shown promising results to relieve the neurosensory disorders of BSSO technique. Objective: The aim of this study was to compare the effects of low-level laser irradiation on the neurosensory complications related to the BSSO technique. Materials and methods: In this prospective, double-blinded clinical trial, 13 patients candidate for BSSO surgery were selected and went under complete clinical neurosensory tests (CNTs) including brush stroke discrimination; two-point discrimination; and contact, thermal, and pinprick discrimination, as well as visual analog scale (VAS) assessments. The laser GaAlAs (820–830 ìm wavelength) irradiation was done for total six sessions after surgery for the patients at one side accidentally, and the opposite site was a control by irradiation of placebo. The values of CNT assessments between the experimental and control sites were analyzed employing Wilcoxon signed-rank test. Results: Higher values of VAS and brush stroke discrimination test were observed in the laser-irradiated sites than in the control sites, although without any significant differences (p > 0.05). Moreover, significantly higher values of pinprick discrimination test were noted in the experimental sites on days 1, 2, 3, 4, 7, 14, 28, and 60 (p < 0.05). The thermal test results were higher in the study sides, and the differences of both sides were significant on days 2, 3, 4, 7, 14, and 28 (p < 0.05). Conclusion: In total, GaAlAs low-level laser irradiation after the BSSO surgery results in both subjective and objective improvements regarding the time and magnitude of return of function; however, in some tests no significant differences were found between laser-irradiated and control areas.

CASE REPORT

Jan Vokurka, Lydie I Holla, Antonin Fassmann

Erbium Laser in Mucocele Surgery

[Year:2015] [Month:] [Volume:5] [Number:2] [Pages:3] [Pages No:50 - 52]

PDF  |  DOI: 10.5005/jp-journals-10022-1073  |  Open Access |  How to cite  | 

Abstract

Mucoceles are benign tumors originating from minor salivary glands of the oral cavity. They are localized most frequently on the lower lip or buccal mucosa. Unintentional lip biting, localized trauma, and orthodontic devices are among the most frequent causes of mucoceles. In this case report, successful treatment of medium-sized mucocele with erbium laser is presented. Benefits as well as potential risks of mucocele surgery are discussed.

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